Improved colorimetric assay for glycosylated hemoglobin.
نویسندگان
چکیده
This colorimetric assay for glycosylated hemoglobin can be performed in 120 min with equipment available in most clinical laboratories. The glucose moiety of glycosylated hemoglobin is converted to 5-hydroxymethylfurfural by heating with oxalic acid for 60 min is an autoclave at 124 degrees C and 124 kPa (18 lb/in.2). The adduct formed by reacting 2-thiobarbituric acid with hydroxymethylfurfural is measured photometrically and results are expressed either as nanomoles of hydroxymethylfurfural or as fructose equivalents. Within-assay and between-assay coefficients of variation were less than 2% and less than 3%, respectively. Comparison of results for 50 patients' specimens as measured by the present assay and as analyzed for hemoglobin A1c by liquid chromatography showed excellent correlation (r = 0.98).
منابع مشابه
Evaluation of a colorimetric method for determination of glycosylated hemoglobin.
We evaluated a colorimetric assay for glycosylated hemoglobin to determine the effects of several variables --oxalic acid concentration, extraneous glucose, hemoglobin concentration, hydrolysis interval, and 5-hydroxymethylfurfural destruction--and the precision. The interference seen when the blood glucose concentration exceeds 2.0 g/L (11 mmol/L) can be eliminated by washing the erythrocytes ...
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 27 5 شماره
صفحات -
تاریخ انتشار 1981